7/24/2023 0 Comments 1902 piano rag by scott joplinAs a result of such performances, the World's Fair was a catalyst for the popularity of American ragtime, which would grow through the end of the century. While in town, he formed a band for which he arranged music and played the cornet. However, Joplin and other black musicians performed in cafes and brothels near the Exposition. It was a gigantic, 690-acre exposition which broke the world record for outdoor event attendance (at about 750,000 people).įor the most part, the World's Fair didn't formally involve African Americans. The Chicago World's Fair commemorated the 400th anniversary of Christopher Columbus's arrival in the New World. We don't know much about Joplin's life during this period, but we do know that in 1893 he went to the World's Fair in Chicago, Illinois. Like his father, he worked as a railway laborer, but in the late 1880's he left that job to travel around the South as a freelance musician. Eventually he started a vocal quartet, taught mandolin and guitar, and learned to play cornet and violin. His interest in music increased as he grew older. As a teenager he attended Lincoln High School and worked as a dance musician. He had perfect pitch and quickly became proficient on piano and banjo. Joplin was an ambitious and serious-mannered kid. To make sure Joplin could keep practicing, Weiss helped them buy a used piano. Giles left his wife and family for another woman, and Florence became a single mother of six children. Weiss tutored Joplin for five years, introducing him to musical styles ranging from operas to folk melodies. The family could not afford to pay him, so he taught for free. Weiss became interested in Scott's talent and offered him private music lessons. At the age of seven, Scott was allowed to play the piano while his mother cleaned in one of her customer's homes.Īround age eleven, he caught the attention of Julis Weiss, a Jewish music teacher who had emigrated tonTexas from Germany. They taught their children a little music. On the plantation where he was born, Giles played the violin at parties and Florence sang and played the banjo. Giles was a railroad laborer, and Florence cleaned homes for a living. The family lived on the Texas border in Texarkana, Arkansas. ![]() Florence was a free-born African American from Kentucky. Giles was an ex-slave from North Carolina. He was the second of six children of Giles Joplin and Florence Gibbons. No one knows exactly when Scott Joplin was born, but it was around November 1868. Before there was the Duke and the Count, there was the King. The ragtimes of Scott Joplin and others influenced the development of early jazz and especially jazz pianists, including Eubie Blake, Thomas "Fats" Waller, Jelly Roll Morton, Duke Ellington, and Count Basie. Like technology, musical styles sometimes persist long past the point of being fashionable. Thus the music roll, invented in the 1880's, has persisted into the twenty-first century. These interfaces often feature a digital music roll, which allows musicians to place every note at a timestamp and play it back with a computer. ![]() ![]() Many film soundtracks are now crafted using this type of software. Digital audio workstations enable users to create music using virtual instruments. Physical music rolls are now outdated technology, but it's interesting to note that their design has influenced modern software. This was a way of sharing music before recorded audio was commonplace. These rolled up pieces of paper, perforated for machines to read, were fed into player pianos and played back as music. ![]() In the early 1900's, that is how many people would have first heard them. You don't need a pianist to hear a rag, if you have a music roll and a player piano. The story of his life is filled with tragedy and suffering, but his art endures. Joplin's music has earned a place in our culture despite a relentless string of professional and personal setbacks. However, as you'll find, in some ways it's surprising we know his name at all. Scott Joplin, a African American man who was called "the king of ragtime", was the most famous composer of piano rags who ever lived.
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7/24/2023 0 Comments Make gif from two imagesIf you set it to a number greater than zero, it would loop that many times and then stop. ![]() Finally, you set loop to 0 (zero), which means that you want the GIF to loop forever. You can set the duration of each frame in milliseconds. You must also set the save_all parameter to True. You also pass in your frames of animation to the append_images parameter. To make that happen, you need to specifically tell Pillow that the format is set to “GIF”. Once you have your Python list of images, you tell Pillow to save() it as a GIF using the first Image in your Python list. Check out How to Resize Photos with Pillow to learn more! If your images are large, you may want to add a step to resize them so that the GIF itself isn’t huge! If you don’t, you are effectively taking in all those images and turning them into one giant file. The next step is to create a Python list of Image objects. Note: You will need to pass in a real path instead of using the placeholder that is in the code above You use glob to search for JPG files in the path that you pass to your make_gif() function. Here you import Python’s glob module and Pillow’s Image class. Then enter the following code: import globįrames = įrame_one.save("my_awesome.gif", format="GIF", append_images=frames, Create a new file and name it gif_maker.py. The first method you will learn about is how to take a series of images (JPGs) and turn them into an animated GIF. You will learn how to use both of these methods to create an animated GIF in this article. You can also use Pillow to draw a series of images and turn that series into a GIF. If you want your GIF to look nice, you should use a tripod or put your camera onto a sturdy surface before taking those photos. If you have a good point-and-shoot or a DSLR camera, you can usually use their high-speed settings to take a series of photos very quickly. You need to have multiple frames of animation to create an animated GIF. Once Pillow is installed, you are ready to create a GIF! Creating an Animation Here is how to do that using pip: python3 -m pip install Pillow Otherwise, you will need to install Pillow. If you are using Anaconda, Pillow is already installed. You can install it from the Python website or use Anaconda. You will need to have Python installed on your machine. You can create your own animated GIFs using the Python programming language and the Pillow package. When you flip the book, the image appears to move. You could even think of it as a flip-book with a stick man that is slightly different on each page. These are then played back kind of like a cartoon is. Animated GIFs are an image type that contains multiple images with slight differences. ![]() ![]() These are part of our wide selection of movie costumes that the whole family will love. You’ll easily be able to help Gru either take over or save the world in one of these bad boys! What about making your Minion costume even more exciting? For both kids and adults, inflatable Minion costumes are a surefire way to get the party started! Everyone will be shocked to see a life-size Minion when you walk into the Halloween party wearing one of these awesome inflatable costumes. We also have plus size Minions costumes for women that look totally picture perfect. Look like you’ve stepped straight out of your favorite movie when you put on one of these adorable women’s Minion costumes. You can dress up in a Minion dress with Gru’s symbol on the front, plus eye goggles, a headband, gloves and socks. Ladies, you never outgrow your love of these adorable characters from Despicable Me. Find the best Minions & Despicable Me Halloween Costumes in every size you can imagine, plus deals that you wont find anywhere else. At that moment a threefoot-tall yellow minion ran through the living room. ![]() Whichever one you choose, you’ll get applause from everyone who sees you. Tomorrow, we'll pick you up a Halloween costume, I told him and he nodded. These officially licensed looks range from an inflatable Minion to Jerry or Dave, or even an easy costume like a Minions shirt and hat. Our official adult Minion costumes let you dress up as your faves from Despicable me. If you want to take a step further, you can even dress your tiny one in a “IT’S SO FLUFFY!!!” toddler Halloween costume (or a unicorn costume to be more specific). From toddlers to adults and anyone in between, Spirit has Minion Halloween costumes for all! Dave, Jorge, and Stuart have always been the three most popular Minions, but since Despicable Me 2, evil Minion costumes or purple minion costumes have been incredibly popular! To really give everyone the full effect of your Despicable Me costume, make sure someone walks around in a Balthazar Bratt Halloween costume and someone else in a Gru costume. be your first choice these are evil mutated versions of the family favorite, yellow minions. There are many different types of minion costume sold by sellers on Etsy. Minions come in all ages, shapes, and sizes. This Halloween, you need an Evil Purple Minion Costume. Spirit Halloween has the best Minion Halloween costumes all in one place so you never have to go looking anywhere else. 7/24/2023 0 Comments Db legends twitter![]()
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Van Heusen 13V0144 Women s Non-Iron Pinpoint Oxford Shirt 45.80 77.86 41 OFF Van Heusen 13V0476 Men Stainshield Essential Shirt 38.47 65. Fabric: 4 oz./yd, 100 cotton oxford Features: Non-iron technology for wrinkle-free good looks and incredible durability Button-down collar Left chest. Buy Van Heusen Womens Wrinkle Free Pinpoint Oxford Shirt, White, Small and other Blouses & Button-Down Shirts at. We carry all the right sizes: Men’s clothing range: XL-8XL. Van Heusen 13V0144 Womens Non-Iron Pinpoint Oxford Shirt. Van Heusen Ladies Non-Iron Pinpoint Oxford Shirt-Because this product is Made to order with custom embellishing, it is not stocked on a shelf. ![]() We have a full selection of shirts, polos, rugbys, t-shirts, tees, casual shirts, graphic tees, hoodies, sweatshirts, sweaters, dress shirts and workwear. Saturday delivery is NOT available.Looking for your Big or Tall size? 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Van Heusen Non-Iron Pinpoint Oxford Womens Shirt 4.0 oz., 100 cotton oxford cloth No-iron technology for wrinkle-free good looks and incredible durability. When you need to look professional, rely on no-iron technology for wrinkle-free wearability with this Van Heusen ladies 4 Oz. Orders placed after the cut off time will be packed on the next business day.įrom time to time there might be warehouse delays, if so that information is specified at the top left of the product page in capitalized red bold underlined text. Orders placed by 3 PM EST should ship the same day. 7/24/2023 0 Comments Smeg blender white![]() Simple to use, we were impressed with the Breville The Fresh and Furious. This entry-level blender combines the brand's sleek styling with power to create a compact blender that's more affordable than other models the brand offers - although the base is made from plastic rather than metal. Still, eye-wateringly expensive price tags may be out of reach for many consumers, except for the Breville The Fresh and Furious (known as Sage the Fresh and Furious in the UK). The best blender 2023īreville offers a range of stylish, durable, and powerful blenders. We've put blenders of all kinds from all the key brands through their paces in real kitchens to discover which ones really deliver the goods, and which ones might be worth paying a premium for.įrom space-savers that are ideal for smaller kitchens through go-anywhere battery-powered portables to the biggest blenders, every model here is a brilliant buy – and if you're new to blending, we've put together some handy guides on how to use a blender and the five best dishes to make in a blender. The only downside to having so much choice is having to choose, and that's why we're here. And because they're so popular, there are tons of different models to choose from. 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In this work, Sf9 cells, used for production of the L1 protein, were lysed under mild stirring in buffer, whereupon the supernatant was centrifuged and filtered to remove cell debris. Large target molecules (> Mr 700 000) pass outside the bead, while smaller molecules can bind to the inner core (Fig 2). The bead has octylamine ligands coupled to the core that is surrounded by an inert outer layer. ![]() Capto Core 700 represents a new generation of chromatography media and is designed and optimized for purification of viruses and other large biomolecules. Protein purity was calculated as the L1 protein-to-total protein ratio and expressed as a percentage. The tentative purity target was set to > 95% as determined by densitometry analysis of electrophoresis gels. Here, we describe a simple procedure to separate the VLPs from host cell-derived impurities to high purity using two orthogonal chromatographic steps: layered-bead size exclusion and anion exchange chromatography. To reach adequate purity, previously established purification processes combines anion exchange, hydrophobic interaction, and hydroxyapatite chromatography. Five L1 protein units form pentamers, which in turn self-assemble into VLPs (Fig 1). Here, the structural L1 protein is expressed in insect Sf9 cells using a baculovirus vector. Nevertheless, the vaccine triggers an immunogenic response that protects against HPV infection. The VLP vaccine lacks the viral nucleic acid and, hence, cannot induce infection. L1 proteins spontaneously self-assemble to hollow VLPs that resemble authentic HPV virons. As papillomaviruses are not easily grown in cell cultures, the vaccine constructs are VLP-based, most commonly with recombinant L1 protein as the structural component. In this work, HPV 58, a subtype commonly occurring in Asia (2), was used as vaccine immunogene. However, vaccines protecting against more genotypes would prevent more cancer forms and be less susceptible to regional variations of the virus. Current vaccines protect against up to four virus genotypes. ![]() To prevent cervical cancer and to reduce the number of treatments for cervical cancer precursors, the World Health Organization (WHO) recommends vaccination of young women against HPV (1). Several genotypes are also classified as low risk and associated with benign lesions and genital warts. More than 111 genotypes of HPV have been described, among which about 30 have been associated with anogenital cancers. HPV is a common sexually transmitted infection in adults. In the second step, Capto Q ImpRes medium allows polishing of the L1 protein with high purity and resolution. In the first step, VLPs are purified in flow-through mode using Capto Core 700, a layered-bead size exclusion medium. A modern, scalable approach, based on two chromatographic steps including novel chromatography media (resins), was used for the purification of L1 protein. ![]() Modified L1 protein was expressed in insect Sf9 cells using baculovirus vector, and allowed to spontaneously assemble into virus like particles (VLPs). This application note describes the results of a project executed by the GE Healthcare BioProcess™ Services team to display proof of principle of a purification procedure for human papillomavirus (HPV) L1 protein, the antigenic component of HPV vaccines. Combo Cleaner Premium 1.3. 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While we’re all for completely homemade recipes, Christmas is also one of the best holidays to get creative with store-bought options. Another way to add intrigue to your cookie selection is to bake up traditional holiday cookies from around the world, like our Middle Eastern ma’amoul, our Polish kolaczki cream cheese cookies, our Italian cuccidati, or our Mexican polvorones. Looking to bowl people over with unexpected flavor? Try our Santa’s trash cookies, our white chocolate cranberry orange biscotti, our panettone cookies, or our molasses cookies. Want to add a little whimsy? There’s SO much you can do with a basic sugar cookie, whether you want to add color to the dough itself, like in our Christmas pinwheels, our candy cane cookies, or our checkerboard cookies or on top in the form of royal icing or frosting, like in our snowflake cookies, our tie-dye Christmas tree cookies, our leg lamp cookies, or our Santa cookies (in the shape of the big man’s face). You’re going to want to start with whatever recipes you think are the most classic-like gingerbread cookies, spritz cookies, chocolate crinkle cookies, peanut butter blossoms.-then go wild. When creating this list, we thought of the perfect Christmas cookie box that’s got a little something for everyone in it. ![]() Check out our favorite Christmas cookies for ideas-don’t forget to make enough to leave out for Santa and his reindeer too. Whether you’re a master baker looking for a new challenge or a kitchen novice looking for easy recipes, you can get in on the holiday baking fun. Luckily, there are myriad opportunities to try them all out, whether you're making them for a party, to give as gifts, or just to enjoy during a cozy night in at home. We get excited every year to combine our old standbys with creative and innovative recipes to share with friends and family. Like to see your post featured in our next roundup? There’s nothing we love more than getting connected with new bloggers and growing together!Įach week we will feature new posts, so be sure to follow us on Pinterest or tag #shetriedwhat on Instagram to get noticed & featured.Īlso, we love making it easier for our readers to try new things, so we are sharing free printable invitations and name cards so you can host your own Christmas cookie exchange this year or next! Click here to get them for FREE.Įnough from me, what are your favorite cookie exchange recipes? I’d love to hear.We can all agree: One of the BEST parts of the holiday season are all the sweet treats, the most classic of which are Christmas cookies. If you follow me on Pinterest, all these incredible recipes are pinned to my STW Recipes board along with other hand-picked recipes from other roundups. Hope you enjoyed our Easy Christmas Cookie Exchange Recipes Roundup! A huge thank you to all of the incredibly talented bloggers whose recipes are featured in this post. ![]() Then, when you tell everyone how easy they are to make, they will be everyone’s new favorite! These red velvet cookies could not be any more looking, so everyone will already adore them. These amazing Red Velvet Chocolate Chip Cake Mix Cookies from Just a Taste only have 4 ingredients! I love Just a Taste’s recipes and this one is no exception. 7/24/2023 0 Comments Awaken online series torrent![]() It has close to 70 million monthly visits, which beats any other recommendation in this guide. When it comes to 1337X, this is one of the most popular TV show torrent sites right now. You can check trending files (daily, weekly, top 100, etc.).īlocked by individual ISPs in some countries. 1337X - Best All-In-One TV Torrents Repository WatchSoMuch has pretty much anything you can imagine, so don’t hesitate to try it out. 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We should also highlight that EZTV has a handy calendar, letting you keep track of freshly released and upcoming content. Therefore, EZTV is your best bet when it comes to downloading TV shows. This repository is about TV shows exclusively, bringing the most extensive library of titles when compared to any other TV torrents site. There's a big reason why we're featuring EZTV first on our list of the best TV shows torrenting sites. Individual episodes, full TV show seasons, TV packs, and more.īy name, date, size, health, and video quality.Ĭalendar of TV shows Countdowns to new episodes Full list of TV shows, and more. ![]() Once the connection is established, start downloading torrents.Choose a server in a country friendly towards torrenting.Launch the VPN and log in using your credentials.Download and install the VPN software on your device.Subscribe to a reputable VPN for torrenting ( we recommend ExpressVPN).Pick one of the best torrent clients before you do anything else. ![]() Here is a step-by-step guide on how to do so: The process of safely downloading TV shows from torrenting sites involves taking certain precautions. Let’s get started! How to Safely Download TV Shows from Torrenting Sites This will consequentially help you make an informed decision when selecting a torrent site for TV shows and stay safe while doing so. In this article, we will provide a comprehensive overview of the best torrent sites for TV shows, including the pain points associated with them and how to overcome limitations with a VPN. VPNs can also help you access geo-restricted content, letting you access the best TV torrents without a hitch. That is why we recommend using the best VPN for torrenting for downloading content from torrent sites as it helps to protect your online privacy and security by encrypting your Internet connection. Furthermore, many popular torrent sites are blocked in certain countries, making it difficult for users in those regions to access the content they want. This can not only harm your device but also put your personal information at risk. More precisely, malware is a known issue in the world of torrents, affecting most P2P repositories. One of the major pain points of torrent sites is their reliability. That’s because many TV torrents are filled with fake files and malware, leading to a potentially dangerous experience. Still, finding the best torrent sites for TV shows can be quite challenging. Torrenting has become a popular method of downloading TV shows, especially since the global streaming market has become so fragmented. |